The aim of the OHEJP subproject LISTADAPT was to investigate the adaptability of Listeria (Listeria monocytogenes) to different ecological niches at the molecular level. This involved comparing both genotypic and phenotypic data from a variety of Listeria strains from the environment, animals, food, and clinical cases in several European countries.
The bacterium Listeria monocytogenes, together with Salmonella and toxin-producing E. coli bacteria (STEC), is the main cause of foodborne infections in the EU, measured by severity of illness and mortality rate. Since 2008, a significant increase in the incidence of listeriosis has been recorded in Europe, highlighting listeriosis as a serious recurrent health problem.
However, the ecology of Listeria monocytogenes is still poorly understood. The high adaptability of some strains to diverse ecological niches also makes the production of high quality, safe food a major challenge. The LISTADAPT project aims to elucidate which genes and molecular mechanisms underlie the adaptation of Listeria to diverse ecological niches.
The project used a combination of next generation sequencing (NGS) technology and phenotypic methods to compare data from a large and balanced number of Listeria strains. Twenty-one partners, including food, environmental, veterinary, and public health laboratories, were involved in generating a dataset of 1,575 strains and genomes of Listeria monocytogenes strains. These were collected in twenty European countries and cover many different habitats and diverse geographic regions.
The LISTADAPT project has made 1,575 new Listeria monocytogenes genomes available to the scientific community. These genomes are representative of the different ecological niches and are now publicly available in the European Nucleotide Archive (ENA).
A subset of Listeria strains was used to test antimicrobial susceptibility to eleven antibiotics and four biocides. Results showed that strains isolated from food had overall higher minimum inhibitory concentrations for quaternary ammonium compounds (QACs) and peracetic acid (PAC) than strains isolated from animals and natural environments. Repeated exposure to QACs resulted in a decrease in susceptibility to ciprofloxacin, a fluoroquinolone antibiotic widely used in human and veterinary medicine and considered a critical antimicrobial agent. Mobile genetic elements were widespread in lineage I and II strains from food, highlighting the high adaptability of these Listeria strains, the nature of tolerance mechanisms, and the need to monitor these genes to reduce the risk of tolerant genotypes in the food industry.
This project was the first to report the presence of a CC121 strain isolated from a dolphin brain. Genomic analyses showed that 16 strains isolated from food were closest to the dolphin strain.
The LISTADAPT project was the first to study a large number of Listeria monocytogenes strains from a wide variety of European habitats. The antimicrobial susceptibility of Listeria to a range of antibiotics and biocides was also tested using a number of strains. The high adaptability of Listeria to ecological niches was also confirmed by the detection of mobile genetic elements in strains isolated from food. In order to keep the spread of Listeria in the food industry and thus the outbreak of listeriosis low, it is of importance to continue to continuously monitor developments in this field.
Benefit of the project
The collection of strains and genomes will be useful for follow-up projects and scientific questions. Detailed characterization of Listeria strains at the phenotypic and genotypic levels will help to assess the true importance of these strains as a source of foodborne infections for public health.
Project acronym: LISTADAPT
Project Management: French Agency for Food, Environmental and Occupational Health & Safety, (ANSES), Maisons-Alfort Laboratory for Food Safety, Unit SEL (Salmonella and Listeria) F-94701 Maisons-Alfort, France
Project Partners: Additional project partners can be found here.
Project management AGES: Mag. Dr. Ariane Pietzka
Funding: One Health EJP - European Union's Horizon 2020 research and innovation programme
Grant Agreement No 773830
Project duration: 11/2017 to 12/2020
Guérin, A., Bridier, A., Le Grandois, P., Sévellec, Y., Palma, F., Félix, B., LISTADAPT Study Group, Roussel, S., Soumet, C., 2021. Exposure to Quaternary Ammonium Compounds Selects Resistance to Ciprofloxacin in Listeria monocytogenes. Pathogens 10, 220. https://doi.org/10.3390/pathogens10020220
Gelbicova, T., Florianova, M., Hluchanova, L., Kalova, A., Korena, K., Strakova, N., Karpiskova, R., 2021. Comparative analysis of genetic determinants encoding cadmium, arsenic and benzalkonium chloride resistance in Listeria monocytogenes of human, food and environmental origin. Front. Microbiol. https://doi.org/10.3389/fmicb.2020.599882
Sévellec, Y., Torresi, M., Félix, B., Palma, F., Centorotola, G., Bilei, S., Senese, M., Terracciano, G., Leblanc, J.-C., Pomilio, F., Roussel, S., 2020. First Report on the Finding of Listeria monocytogenes ST121 Strain in a Dolphin Brain. Pathogens, 9, 802. https://doi.org/10.3390/pathogens9100802
Torresi, M., Orsini, M., Acciari, V., Centorotola, G., Di Lollo, V., Di Domenico, M., Bianchi, D. M., Ziba, M. W., Tramuta, C., Cammà, C., Pomilio, F., 2020. Genetic Characterization of a Listeria monocytogenes Serotype IVb Variant 1 Strain Isolated from Vegetal Matrix in Italy. Microbiology resource announcements, 9, 33. https://doi.org/10.1128/MRA.00782-20
Pietzka, A., Murer, A., Lennkh, A., Hauser, K., Vötsch, K., Springer, B., Allerberger, F., Ruppitsch, W., 2021. Draft genomes of two Listeria monocytogenes strains isolated from invasive snails Arion vulgaris in Austria, 2019. Microbiol Resour Announc 10. https://doi.org/10.1128/MRA.00375-21
Félix, B., Sévellec, Y., Palma, F., Felten, A., Radomski, N., Mallet, L., Blanchard, Y., Leroux, A., Soumet, C., Bridier, A., Piveteau, P., Ascensio, E., Hébraud, M., Karpíšková, R., Gelbíčová, T., Torresi, M., Pomilio, F., Cammà, C., Di Pasquale, A., Skjerdal, T., Pietzka, A., Ruppitsch, W., Ricão Canelhas, M., Papić, B., Hurtado, A., Wullings, B., Bulawova, H., Castro, H., Lindström, M., Korkeala, H., Šteingolde, Z., Kramarenko, T., Cabanova, L., Szymczak, B., Gareis, M., Oswaldi, V., Guedes, H., Marti, E., Seyfarth, A.-M., Leblanc, J.-C., Guillier, L., Roussel, S. A, 2022. European-wide dataset to uncover adaptive traits of Listeria monocytogenes to diverse ecological niches. Sci Data. https://doi.org/10.1038/s41597-022-01278-6
Last updated: 05.07.2022